programmable horizontal puller model p-87 Search Results


horizontal puller p87  (Sutter Instrument Company)
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Sutter Instrument Company horizontal puller p87
Horizontal Puller P87, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal electrode puller p-87  (Sutter Instrument Company)
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Sutter Instrument Company horizontal electrode puller p-87
Horizontal Electrode Puller P 87, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal puller  (Sutter Instrument Company)
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Sutter Instrument Company horizontal puller
Horizontal Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vertical puller  (Narishige inc)
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Narishige inc vertical puller
Vertical Puller, supplied by Narishige inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal electrode puller  (Sutter Instrument)
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Sutter Instrument horizontal electrode puller
Horizontal Electrode Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal puller brown flaming p–87  (Sutter Instrument Company)
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Sutter Instrument Company horizontal puller brown flaming p–87
Horizontal Puller Brown Flaming P–87, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal multistep puller  (Sutter Instrument Company)
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Sutter Instrument Company horizontal multistep puller
Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline <t>(horizontal</t> dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.
Horizontal Multistep Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sutter p 87 flaming brown micropipette horizontal puller  (Narishige inc)
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Narishige inc sutter p 87 flaming brown micropipette horizontal puller
Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline <t>(horizontal</t> dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.
Sutter P 87 Flaming Brown Micropipette Horizontal Puller, supplied by Narishige inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sutter p 87 flaming brown micropipette horizontal puller - by Bioz Stars, 2026-06
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horizontal multistep puller p87  (Sutter Instrument Company)
90
Sutter Instrument Company horizontal multistep puller p87
Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline <t>(horizontal</t> dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.
Horizontal Multistep Puller P87, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horizontal multistep puller p87/product/Sutter Instrument Company
Average 90 stars, based on 1 article reviews
horizontal multistep puller p87 - by Bioz Stars, 2026-06
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horizontal electrode puller sutter instruments p87  (Sutter Instrument Company)
90
Sutter Instrument Company horizontal electrode puller sutter instruments p87
Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline <t>(horizontal</t> dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.
Horizontal Electrode Puller Sutter Instruments P87, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horizontal electrode puller sutter instruments p87 - by Bioz Stars, 2026-06
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programmable puller p87  (Sutter Instrument Company)
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Sutter Instrument Company programmable puller p87
Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline <t>(horizontal</t> dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.
Programmable Puller P87, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/programmable puller p87/product/Sutter Instrument Company
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programmable puller p87 - by Bioz Stars, 2026-06
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horizontal micropipette puller  (Sutter Instrument)
90
Sutter Instrument horizontal micropipette puller
Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline <t>(horizontal</t> dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.
Horizontal Micropipette Puller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline (horizontal dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.

Journal: The Journal of Neuroscience

Article Title: Direct and Indirect Excitation of Laterodorsal Tegmental Neurons by Hypocretin/Orexin Peptides: Implications for Wakefulness and Narcolepsy

doi: 10.1523/JNEUROSCI.22-07-02862.2002

Figure Lengend Snippet: Hcrt/Orx produced an increase in glutamatergic synaptic activity, an inward current, and an increase in membrane current noise. A1, Whole-cell recordings of membrane current at −60 mV in the presence of bicuculline (10 μm) and strychnine (2.5 μm) indicate that Hcrt/Orx-A (1 μm) application produced a large increase in sEPSC activity and an inward shift of the baseline current that reversed after a washout of ∼20 min. A2,Arrow indicates the neuron whose currents are shown inA–C. The neuron was filled with biocytin during the recording and visualized with avidin–Texas Red epifluorescence.A3, The same neuron was also visible with FITC immunofluorescence for bNOS. Scale bar (inA2):A2,A3, 25 μm.B1, An expanded segment of thetrace in A1 before Hcrt/Orx application (labeled B1 inA1).B2, Expanded segment fromA1 after Hcrt/Orx-A application illustrates the greater number of sEPSCs and the inward current shift compared with baseline (horizontal dashed line).B3, These effects recovered by 20 min after the end of the Hcrt/Orx application. C, Cumulative distributions of sEPSC amplitudes (left;Amp) and intervals (right;Int) from before (thin line) and after (thick line) Hcrt/Orx-A application. Kolmogorov–Smirnov statistics demonstrated that Hcrt/Orx-A significantly increased both amplitude and frequency of sEPSCs. D, Population means of sEPSC amplitudes (black bars) and inter-event intervals (white bars) from 15 LDT neurons expressed as percentage of control values. Each neuron showed a significant reduction in the inter-event interval distribution after 1 μm Hcrt/Orx-A. For six of these cells, sufficient recording time was available to observe a recovery after washout of the peptide. E, EPSCs recorded in the presence of bicuculline and strychnine (left; BS) were entirely abolished by the addition of DNQX and APV (middle; DABS). Subsequent application of Hcrt/Orx (300 nm) produced an inward current but no additional PSCs. A substantial increase in current noise accompanied the inward current. Arrows point to expanded sections of the trace before and after Hcrt/Orx to illustrate the noise at higher time resolution. The time scale in themiddle also applies to the left. Note the different time scales in the right.

Article Snippet: Giga-seal whole-cell recordings ( Hamill et al., 1981 ) were made with pipettes pulled from 1.5-mm-diameter glass capillary tubing (Corning 7052; A&M Systems, Carlsborg, WA) using a horizontal multistep puller (P87; Sutter Instruments, Novato, CA).

Techniques: Produced, Activity Assay, Membrane, Avidin-Biotin Assay, Immunofluorescence, Labeling, Control